Coding
Part:BBa_K3619012:Design
Designed by: Isak Ingerholt Group: iGEM20_Lund (2020-09-13)
cDNA encoding the antimicrobial peptide Temporin-1Ta
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence in codon optimized for expression in E.coli K12.
Source
Temporin-1Ta originates from Rene temporia. DNA sequence was obtained through back translation of peptide found in the Uniprot record P56917.
References
1. https://www.uniprot.org/uniprot/P56917
2. https://webs.iiitd.edu.in/raghava/cancerppd/refpdf/9022710.pdf